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GBI-LNMA_2024_image_analysis_and_image_repositories_course

GBI-LNMA 2024 course: Image analysis and data reuse through image repositories

https://globalbioimaging.org/international-training-courses/gbi-lnma-2024-course

Programme

Day 1: Monday, 10th of June

8:30 - 9:00 Registration at the Institute of Biotechnology (Instituto de Biotecnología)
9:00 - 9:30 Welcome by the director of the Institute of Biotechnology - Laura Palomares (National Autonomous University of Mexico)
9:30 - 11:00 Introduction to digital images - Trainer: Rocco D’Antuono (Francis Crick Institute), Helper: Stefania Marcotti (King's College London)
11:00 - 11:30 Coffee break
11:30 - 13:00 Introduction to digital images (continued) - Trainer: Rocco D’Antuono (Francis Crick Institute), Helper: Stefania Marcotti (King's College London)
13:00 - 14:00 Lunch
14:00 - 16:30 Using ImageJ/FIJI via a graphical user interface (GUI) - Trainer: Stefania Marcotti (King's College London), Helpers: Rocco D’Antuono (Francis Crick Institute), Christopher Wood
16:30 - 17:00 Participants are split into (thematic) groups (4-5 participants per group) - Participants & instructors of the course
17:00 - 17:45 Visit to the Microscopy Facility at the Institute of Biotechnology -

A visit to the Laboratorio Nacional de Microscopía Avanzada (National Laboratory of Advanced Microscopy) to explore its facilities and imaging technologies

Day 2: Tuesday, 11th of June

9:00 - 10:30 Introduction to ImageJ macro language - Trainer: Stefania Marcotti (King's College London), Helper: Rocco D’Antuono (Francis Crick Institute)
10:30 - 11:00 Coffee break
11:00 - 13:00 Introduction ImageJ macro language (continued) - Trainer: Stefania Marcotti (King's College London), Helper: Rocco D’Antuono (Francis Crick Institute)
13:00 - 14:00 Lunch
14:00 - 15:00 Invited scientific lecture focused on image analysis
15:00 - 17:00 Work in thematic groups (4-5 participants per group) - Participants & instructors of the course
17:00 - 17:45 Presentation of Folkloric Dance by the University of the State of Morelos

A unique performance by a local folkloric dance group

Day 3: Wednesday, 12th of June

9:00 - 10:30 Introduction to Python programming (Session #1) - Trainer: Sebastian Gonzalez Tirado (European Molecular Biology Laboratory), Helpers: Rocco D’Antuono (Francis Crick Institute), Stefania Marcotti (King's College London), Adan Guerrero, (National Autonomous University of Mexico)
10:30 - 11:00 Coffee break
11:00 - 13:00 Introduction to Python programming (Session #1 continued) - Trainer: Sebastian Gonzalez Tirado (European Molecular Biology Laboratory), Helpers: Rocco D’Antuono (Francis Crick Institute), Stefania Marcotti (King's College London), Adan Guerrero (National Autonomous University of Mexico)
13:00 - 14:00 Lunch
14:00 - 15:00 Managing your image data for the long term: FAIR data, REMBI metadata and the BioImage Archive - Speaker: Matthew Hartley (European Bioinformatics Institute)
15:00 - 17:00 Work in thematic groups (4-5 participants per group) - Participants & instructors of the course
17:00 - 17:45 Venom House Tour - Tour of the facility that includes venomous reptiles (snakes & lizards) that are used in antivenom research and production

Day 4: Thursday, 13th of June

9:00 - 10:30 Introduction to Python programming (Session #2) - Trainer: Sebastian Gonzalez Tirado (European Molecular Biology Laboratory), Helpers: Rocco D’Antuono (Francis Crick Institute), Stefania Marcotti (King's College London), Adan Guerrero (National Autonomous University of Mexico)
10:30 - 11:00 Coffee break
11:00 - 13:00 Introduction to Python programming (Session #2 continued) - Trainer: Sebastian Gonzalez Tirado (European Molecular Biology Laboratory), Helpers: Rocco D’Antuono (Francis Crick Institute), Stefania Marcotti (King's College London), Adan Guerrero (National Autonomous University of Mexico)
13:00 - 14:00 Lunch
14:00 - 17:00 BioImage Archive image data repository workshop - Trainer: Aybuke Kupcu Yoldas (European Bioinformatics Institute)
17:00 - 17:45 Visit to the Museum of Biology and Photography Exhibition

Day 5: Friday, 14th of June

9:00 - 10:30 Napari viewer - Trainer: Rocco D’Antuono (Francis Crick Institute), Helpers: Adan Guerrero (National Autonomous University of Mexico)
10:30 - 11:00 Coffee Coffee break
11:00 - 13:00 Napari viewer & plugins - Trainer: Rocco D’Antuono (Francis Crick Institute), Helpers: Adan Guerrero (National Autonomous University of Mexico)
13:00 - 14:00 Lunch
14:00 - 15:00 Napari superres & zelda plugins - Trainers: Rocco D’Antuono (Francis Crick Institute) & Julian Mejia Morales (National Autonomous University of Mexico)
15:00 - 15:30 Building reusable bioimage analysis workflows with Nextflow - Speaker: Sebastian Gonzalez Tirado (European Molecular Biology Laboratory)
15:30 - 17:00 Presentations from thematic groups (4-5 participants per group) - Participants of the course
17:30 Closing Dinner - Dinner featuring local cuisine and final opportunity to network with participants and the faculty

Software installation

ImageJ / FIJI

  1. Download FIJI from here.

  2. To avoid any permissions issues, install FIJI is in your home directory:

    • PC: C:\users\<your user name>
    • Mac: /Users/<your user name>

    WARNING: FIJI must be installed in a location where it has write permission - otherwise, it cannot update itself

  3. Start FIJI and allow the updater to run:

  4. (Optional) If the updater does not run automatically, select Help > Update:

  5. If FIJI produces any error messages, it is most likely because it does not have the necessary permissions to update itself - return to step #2 and double-check the location of the installation.

Jupyter Notebook or Jupyter Lab

napari

use Anaconda Prompt or other terminal to create a conda environment (make sure you have no other existing "mynapari-env")

conda create -y -n mynapari-env python=3.8  
conda activate mynapari-env
conda install napari pyqt  

napari plugins showcase:

How to install napari-superres

conda create -y -n np-superres -c conda-forge python=3.9
conda activate np-superres
conda install -c conda-forge napari pyqt ipywidgets git

use graphical installation

or get the latest developments with

pip install git+https://github.com/RoccoDAnt/napari-superres.git

More info at https://github.com/RoccoDAnt/napari-superres

How to install napari-zelda

conda create -y -n np-zelda python=3.8
conda activate napari-env
conda install napari pyqt git

use graphical installation

  1. Plugins / Install/Uninstall Package(s)

  1. Choose ZELDA

or get the latest developments with

pip install git+https://github.com/RoccoDAnt/napari-zelda.git

More info at https://github.com/RoccoDAnt/napari-zelda

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