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java.lang.IllegalStateException: Smith-Waterman alignment failure. Cigar = 219M with reference length 219 but expecting reference length of 275 ref = TGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCACGAGCGCCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCACGAGCGCCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCACGAGCACCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCATGAGCACCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTCTGACTTCCACGCTGG path TGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCACGAGCGCCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCACGAGCACCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTGGCCTCACGAGCACCGCTGGGTGCCTGAGCACCTGCCCCACCAGAGCGCCACTGCTCTGACTTCCACGCTGG
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.readthreading.ReadThreadingAssembler.findBestPaths(ReadThreadingAssembler.java:354)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.readthreading.ReadThreadingAssembler.assembleKmerGraphsAndHaplotypeCall(ReadThreadingAssembler.java:196)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.readthreading.ReadThreadingAssembler.runLocalAssembly(ReadThreadingAssembler.java:146)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyBasedCallerUtils.assembleReads(AssemblyBasedCallerUtils.java:269)
at org.broadinstitute.hellbender.tools.walkers.mutect.Mutect2Engine.callRegion(Mutect2Engine.java:226)
at org.broadinstitute.hellbender.tools.walkers.mutect.Mutect2.apply(Mutect2.java:299)
at org.broadinstitute.hellbender.engine.AssemblyRegionWalker.processReadShard(AssemblyRegionWalker.java:200)
at org.broadinstitute.hellbender.engine.AssemblyRegionWalker.traverse(AssemblyRegionWalker.java:173)
at org.broadinstitute.hellbender.engine.GATKTool.doWork(GATKTool.java:1048)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:139)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:191)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:210)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:163)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:206)
at org.broadinstitute.hellbender.Main.main(Main.java:292)
I ran sarek with
mutect2
and ran into this:Looks like this is a known issue (broadinstitute/gatk#6533) and fixed in the new release 4.1.7 : https://github.com/broadinstitute/gatk/releases
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