diff --git a/cli/tsc/dts/lib.deno.ns.d.ts b/cli/tsc/dts/lib.deno.ns.d.ts
index e47a2d942d75fd..319b1da4554e58 100644
--- a/cli/tsc/dts/lib.deno.ns.d.ts
+++ b/cli/tsc/dts/lib.deno.ns.d.ts
@@ -2530,17 +2530,6 @@ declare namespace Deno {
     [Symbol.dispose](): void;
   }
 
-  /**
-   * The Deno abstraction for reading and writing files.
-   *
-   * @deprecated This will be removed in Deno 2.0. See the
-   * {@link https://docs.deno.com/runtime/manual/advanced/migrate_deprecations | Deno 1.x to 2.x Migration Guide}
-   * for migration instructions.
-   *
-   * @category File System
-   */
-  export const File: typeof FsFile;
-
   /** Gets the size of the console as columns/rows.
    *
    * ```ts
diff --git a/ext/fs/30_fs.js b/ext/fs/30_fs.js
index 35bf0e712eabb7..f979badb970b3a 100644
--- a/ext/fs/30_fs.js
+++ b/ext/fs/30_fs.js
@@ -786,8 +786,6 @@ function checkOpenOptions(options) {
   }
 }
 
-const File = FsFile;
-
 function readFileSync(path) {
   return op_fs_read_file_sync(pathFromURL(path));
 }
@@ -950,7 +948,6 @@ export {
   cwd,
   fdatasync,
   fdatasyncSync,
-  File,
   FsFile,
   fsync,
   fsyncSync,
diff --git a/runtime/js/99_main.js b/runtime/js/99_main.js
index d0171acd27e02d..913761f6a82a87 100644
--- a/runtime/js/99_main.js
+++ b/runtime/js/99_main.js
@@ -801,7 +801,6 @@ function bootstrapMainRuntime(runtimeOptions, warmup = false) {
     if (internals.future) {
       delete globalThis.window;
       delete Deno.Buffer;
-      delete Deno.File;
       delete Deno.FsFile.prototype.rid;
       delete Deno.funlock;
       delete Deno.funlockSync;
@@ -965,7 +964,6 @@ function bootstrapWorkerRuntime(
 
     if (internals.future) {
       delete Deno.Buffer;
-      delete Deno.File;
       delete Deno.FsFile.prototype.rid;
       delete Deno.funlock;
       delete Deno.funlockSync;
diff --git a/tests/specs/future/runtime_api/main.js b/tests/specs/future/runtime_api/main.js
index 26a75373eadede..afaa4e57bb1e45 100644
--- a/tests/specs/future/runtime_api/main.js
+++ b/tests/specs/future/runtime_api/main.js
@@ -1,6 +1,5 @@
 console.log("window is", globalThis.window);
 console.log("Deno.Buffer is", Deno.Buffer);
-console.log("Deno.File is", Deno.File);
 console.log(
   "Deno.FsFile.prototype.rid is",
   Deno.openSync(import.meta.filename).rid,
diff --git a/tests/specs/future/runtime_api/main.out b/tests/specs/future/runtime_api/main.out
index 0aa4e1f7c982b9..d629b13b40722c 100644
--- a/tests/specs/future/runtime_api/main.out
+++ b/tests/specs/future/runtime_api/main.out
@@ -1,6 +1,5 @@
 window is undefined
 Deno.Buffer is undefined
-Deno.File is undefined
 Deno.FsFile.prototype.rid is undefined
 Deno.funlock is undefined
 Deno.funlockSync is undefined
diff --git a/tests/unit/files_test.ts b/tests/unit/files_test.ts
index c9c3c011003b55..7b939d1ece0731 100644
--- a/tests/unit/files_test.ts
+++ b/tests/unit/files_test.ts
@@ -1,7 +1,5 @@
 // Copyright 2018-2024 the Deno authors. All rights reserved. MIT license.
 
-// deno-lint-ignore-file no-deprecated-deno-api
-
 import {
   assert,
   assertEquals,
@@ -24,7 +22,6 @@ Deno.test(
   async function filesCopyToStdout() {
     const filename = "tests/testdata/assets/fixture.json";
     using file = await Deno.open(filename);
-    assert(file instanceof Deno.File);
     assert(file instanceof Deno.FsFile);
     assert(file.rid > 2);
     const bytesWritten = await copy(file, Deno.stdout);