diff --git a/pkgs/development/libraries/gdk-pixbuf/default.nix b/pkgs/development/libraries/gdk-pixbuf/default.nix
index 24c8558c186e9..6f058718ab942 100644
--- a/pkgs/development/libraries/gdk-pixbuf/default.nix
+++ b/pkgs/development/libraries/gdk-pixbuf/default.nix
@@ -19,6 +19,7 @@
 , doCheck ? false
 , makeWrapper
 , lib
+, callPackage
 , withIntrospection ? (stdenv.buildPlatform == stdenv.hostPlatform)
 , gobject-introspection
 }:
@@ -140,6 +141,7 @@ stdenv.mkDerivation rec {
 
     tests = {
       installedTests = nixosTests.installed-tests.gdk-pixbuf;
+      multiple-module-files = callPackage ./multiple-module-files-test {};
     };
 
     # gdk_pixbuf_moduledir variable from gdk-pixbuf-2.0.pc
diff --git a/pkgs/development/libraries/gdk-pixbuf/multiple-module-files-test/default.nix b/pkgs/development/libraries/gdk-pixbuf/multiple-module-files-test/default.nix
new file mode 100644
index 0000000000000..f56c0e509f228
--- /dev/null
+++ b/pkgs/development/libraries/gdk-pixbuf/multiple-module-files-test/default.nix
@@ -0,0 +1,34 @@
+# Simple tests for multiple module files functionality.
+# In the future, would like to test precedence, more than two files, unexpected inputs/fuzzing.
+{ runCommand, gdk-pixbuf, librsvg}:
+runCommand "pixbuf-mmf-test" {} ''
+  mkdir -p $out
+  set +e
+
+  function test {
+    modulepath=$1
+    testfile=$2
+    GDK_PIXBUF_MODULE_FILE=$modulepath ${gdk-pixbuf}/bin/gdk-pixbuf-thumbnailer $testfile $out/$(basename $testfile) > /dev/null
+  }
+
+  test ${gdk-pixbuf}/${gdk-pixbuf.cacheFile} ${./sample.svg}
+  if ! (($?)); then
+    >&2 echo "This shouldn't happen? SVG parsing succeeded even though librsvg wasn't supplied."
+    exit 1
+  fi
+
+  test ${gdk-pixbuf}/${gdk-pixbuf.cacheFile}:${librsvg}/${gdk-pixbuf.cacheFile} ${./sample.svg}
+  if (($?)); then
+    >&2 echo "Test failed."
+    exit 1
+  fi
+
+  test ${librsvg}/${gdk-pixbuf.cacheFile}:${gdk-pixbuf}/${gdk-pixbuf.cacheFile} ${./sample.svg}
+  if (($?)); then
+    >&2 echo "Test failed."
+    exit 1
+  fi
+
+  set -e
+  exit 0
+''
diff --git a/pkgs/development/libraries/gdk-pixbuf/multiple-module-files-test/sample.svg b/pkgs/development/libraries/gdk-pixbuf/multiple-module-files-test/sample.svg
new file mode 100644
index 0000000000000..22bd85d520bc4
--- /dev/null
+++ b/pkgs/development/libraries/gdk-pixbuf/multiple-module-files-test/sample.svg
@@ -0,0 +1,79 @@
+<?xml version="1.0" encoding="UTF-8" standalone="no"?>
+<!-- Created with Inkscape (http://www.inkscape.org/) -->
+
+<svg
+   width="500"
+   height="500"
+   viewBox="0 0 132.29166 132.29167"
+   version="1.1"
+   id="svg5"
+   inkscape:version="1.1.1 (3bf5ae0d25, 2021-09-20)"
+   sodipodi:docname="sample.svg"
+   xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape"
+   xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd"
+   xmlns="http://www.w3.org/2000/svg"
+   xmlns:svg="http://www.w3.org/2000/svg">
+  <sodipodi:namedview
+     id="namedview7"
+     pagecolor="#ffffff"
+     bordercolor="#666666"
+     borderopacity="1.0"
+     inkscape:pageshadow="2"
+     inkscape:pageopacity="0.0"
+     inkscape:pagecheckerboard="0"
+     inkscape:document-units="mm"
+     showgrid="false"
+     units="px"
+     width="500px"
+     inkscape:zoom="0.64052329"
+     inkscape:cx="272.4335"
+     inkscape:cy="254.47943"
+     inkscape:window-width="1920"
+     inkscape:window-height="1043"
+     inkscape:window-x="0"
+     inkscape:window-y="0"
+     inkscape:window-maximized="1"
+     inkscape:current-layer="layer1" />
+  <defs
+     id="defs2" />
+  <g
+     inkscape:label="Layer 1"
+     inkscape:groupmode="layer"
+     id="layer1">
+    <circle
+       style="fill:none;stroke:#000000;stroke-width:0.529167"
+       id="path850"
+       cx="38.309357"
+       cy="-40.626446"
+       r="29.084583"
+       transform="scale(1,-1)" />
+    <circle
+       style="fill:none;stroke:#000000;stroke-width:0.529167"
+       id="circle932"
+       cx="92.564011"
+       cy="-79.005188"
+       r="29.084583"
+       transform="scale(1,-1)" />
+    <circle
+       style="fill:none;stroke:#000000;stroke-width:0.529167"
+       id="circle934"
+       cx="40.876545"
+       cy="-90.781021"
+       r="29.084583"
+       transform="scale(1,-1)" />
+    <circle
+       style="fill:none;stroke:#000000;stroke-width:0.529167"
+       id="circle936"
+       cx="81.925743"
+       cy="-43.756771"
+       r="29.084583"
+       transform="scale(1,-1)" />
+    <circle
+       style="fill:none;stroke:#000000;stroke-width:0.529167"
+       id="circle938"
+       cx="58.809753"
+       cy="-77.635269"
+       r="29.084583"
+       transform="scale(1,-1)" />
+  </g>
+</svg>